5/16/2023 0 Comments Srdx figure kotona![]() Candidates are the MYB domain-containing pseudo response regulators APRR2 and APRR4, which show in their DNA binding domains similarity to B-type ARRs, but nothing is known about the function of these APRRs (Makino et al., 2000). However, we cannot completely exclude that part of the changes in phenotype and gene regulations caused by ARR1- SRDX are off-target effects due to interference with other related transcription factors. It is likely, therefore, that ARR1-SRDX suppresses the activity of most if not all B-type ARRs, which is supported by the results of the protoplast transactivation assay (Fig. B-type ARRs are similar but not iden- tical (Imamura et al., 2003). ![]() The significance codes are based on the uncorrected P value for each gene. The cytokinin response of genes with a significant I (interaction effect) is dependent on the genotype. G (genotype effect) denotes whether the average expression of the respective gene in line ARR1-S-8 differs significantly from that in the wild type. ![]() C (cytokinin effect) represents the responsiveness of the respective gene to cytokinin treatment. The table in the lower right corner shows the significance of differences in gene expression. Interaction plots show the relative normalized expression values for all seven A-type ARR genes present on the CATMA V2.4 array plotted against the time points of cytokinin treatment: BA0 (control), BA15 (15 min of BA treatment), and BA120 (120 min of BA treatment). B, Interaction plots of A-type ARR genes. ![]() The ACTIN2 gene was used as a loading control. Northern blots were hybridized with a probe specific for the cytokinin response genes ARR5 and ARR7. Total RNA was isolated from 5-d-old wild-type and 35S : ARR1-SRDX seedlings before treatment and after 15, 30, and 120 min of treatment with 5 m M BA. A, Northern-blot analysis of ARR5 and ARR7 transcript levels. 35S : ARR1-SRDX gene expression dampens the early cytokinin response. ![]()
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